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(PDF) Application of Fungal Biofilm Supported on …

Pseudomonas pseudoalcaligenes and biofilm supported on activated carbon were used for adsorption of acid blue 25 (AB 25). The effect of initial dye concentration (0-300 mg/L), contact time (0-240 ...

''Nano on micro'' hierarchical porous all carbon structures ...

2018-11-6 · Micron long carbon nanofibers (CNFs) were grown on porous carbon beads to give an active surface for rapid immobilization of guest molecules. The fabrication of nanostructures using a catalytic route involving chemical vapour deposition on a …

Application of Fungal Biofilm Supported on Activated ...

2.3. Biofilm Preparation The biofilm was prepared by shaking method. Granulated activated carbon (AC) (0.5g) and AUMC-050 cells (0.5g) were mixed in deionized water (500 ml) and incubated at 37 °C for 72 hours under shaking followed by centrifugation, the pellet was …

Activated Carbon Interphase Effect on Biofilm and Total ...

2018-3-12 · The study treatment evaluated in this study is Zorflex® dressing (Chemviron Carbon Cloth Carbon, West Midlands, United Kingdom; a division of Calgon Carbon Corporation, Pittsburgh, PA). According to the Instructions for Use for the product, it is a low-adherent, pure activated carbon cloth dressing that highly conforms to the body ...

Cryptococcus neoformans biofilm formation depends on ...

2007-5-18 · Biofilm formation is a well-organized process that depends on effects of the surface, conditioning films on the surface, characteristics of the medium, and properties of the microbial cell . In this study, we investigated the effect of these factors on cryptococcal biofilm formation and compared the susceptibilities of mature C. neoformans ...

Biofilm form and function: carbon availability affects ...

2010-10-29 · The small amount of carbon required to maintain the yield of planktonic cells may explain how the pioneer, surface-attached cells are able to form a multi-layered biofilm through growth and cell division, while simultaneously yielding planktonic cells to the environment as early as 6 h after initial attachment (Bester et al. 2009) as well as ...

(PDF) Portable hyperspectral fluorescence imaging system ...

A portable hyperspectral fluorescence imaging system was used to evaluate potential detection of microbial biofilm on stainless steel typically used in the manufacture of food processing equipment.

Application of Fungal Biofilm Supported on Activated ...

2015-6-19 · J. Rivera‐Utrilla, I. Bautista‐Toledo, M.A. Ferro‐García, C. Moreno‐Castilla, Activated carbon surface modifications by adsorption of bacteria and their effect on aqueous lead adsorption, Journal of Chemical Technology and biotechnology, 76 (2001) 1209-1215.

Visible Light-Activated Carbon Dots for Inhibiting Biofilm ...

2021-11-18 · This study aimed to address the significant problems of bacterial biofilms found in medical fields and many industries. It explores the potential of classic photoactive carbon dots (CDots), with 2,2′-(ethylenedioxy)bis (ethylamine) (EDA) for dot surface functionalization (thus, EDA-CDots) for their inhibitory effect on B. subtilis biofilm formation and the inactivation of B. subtilis cells ...

Fluorescent quenching for biofilm extracellular polymeric ...

2012-5-1 · The Cu(II) binding properties of loosely bound extracellular polymeric substances (LBEPSs) and tightly bound EPSs (TBEPSs) extracted from biofilm samples at two apparent molecular weight (AMW) ranges, >14 kDa and 1–14 kDa, were investigated using three-dimensional excitation–emission matrix (EEM) fluorescence spectroscopy.The protein-like and aromatic protein fluorescence peaks …

Biofilm microbial composition changes due to different ...

2019-6-11 · Abstract Activated carbon cloths (ACCs) were used as biofilms supports in the anaerobic biotransformation of 4-nitrophenol (4NP). As received ACC material (AW) was oxidized with HNO3 (OX) and then functionalized with anthraquinone-2,6-disulfonate (AQ). The three ACCs were packed in hybrid UASB reactors and seeded with anaerobic granular sludge for biotransformation experiments. The …

Visualization of active biomass distribution in a BGAC ...

A favorable microenvironment for biofilm growth on GAC particles was shown using green fluorescent protein (GFP) as a marker for a phenol degrading bacterium, Pseudomonas putida F1. The dispersion of P. putida F1 in a biofilm covering granulated activated carbon (GAC) particles was monitored and compared to a biofilm on non-activated granular ...

Response of hyporheic biofilm metabolism and …

to surface waters but existing in the dark, are reliant on dissolved organic matter (DOM) downwelling from surface waters, or supply of particulate organic matter, for energy (Findlay & Sobczak 2000). In many aquatic ecosystems, organic matter inputs are enriched in carbon relative to nitrogen and phos-phorus.

Colonization of Granular Activated Carbon Media Filters …

2015-2-1 · Colonization of Granular Activated Carbon Media Filters By Legionella and Heterotrophic Bacterial Cells by ... Significant increase in the Legionella was observed in column''s inner surface biofilm (0.25 logs up to 0.52 logs) and on GAC particles (0.42 logs up to 0.63 logs) after 2 ... Fluorescent gfp-strain of Legionella Glowing Green Under UV

Molecular and microbial insights towards understanding …

2019-12-1 · Moreover, the microbial communities in the biofilm on the surface of GAC as well as the suspened sludge in the liquid were investigated by high-throughput sequencing of 16S rRNA genes. These results would provide an in-depth understanding on the effects of GAC on the AD of HTLWW from molecular and microbial aspects. 2. Material and methods2.1.

Optimization of Microbial Adsorption (Biofilm Creation) …

2021-3-26 · Optimization of Microbial Adsorption (Biofilm Creation) on Activated Carbon by Surface Modification of Substrate 2013 ii | P a g e Bacteria/mm2 Activated Carbon) and 7 (1.58 x 105 Bacteria/mm2 Activated Carbon) were achieved. The greatest attachment was seen when the activated carbon was charged to a pH of 7.

Biofilms: Microbial Life on Surfaces

A biofilm is an assemblage of surface-associated microbial cells that is enclosed in an extracellular polymeric substance matrix. Van Leeuwenhoek, using his simple microscopes, first observed microorganisms on tooth surfaces and can be credited …

Regeneration of the power performance of cathodes …

2016-7-1 · These changes include increasing the biofilm thickness as well as precipitation and adsorption of chemical compounds on the electrode surface, . The biofilm activity on the cathode was widely used as an approach for increasing the oxygen reduction rates, when pure phototrophic cultures or aerobic activated sludge were used as the biocatalyst, .

Extraction and structural characteristics of extracellular ...

2010-10-1 · Two laboratory-scale continuously stirred tank reactors were operated for 4 months to develop a nitrifying biofilm and activated sludge using sodium bicarbonate as the sole inorganic carbon source.The laboratory-scale continuously aerated reactor is cylindrical and is made of transparent PVC plastic with volume of 6 L.The reactor is 20 cm high and 19.5 cm in diameter.

Patchy Biofilm Coverage Can Explain the Potential ...

An adsorbing biofilm carrier, like granular activated carbon (GAC), can be the source of an extra flux of pollutant to the biofilm in addition to the bulk liquid.

Optimization of microbial adsorption (Biofilm Creation) on ...

2014-2-25 · However establishing the biofilm on the activated carbon was a lengthy process in terms of the time it took the bacteria to attach to the various substrates (Activated Carbon and Cellulose) and form flocculants. The formation of such flocculants substantially increases the reaction surface and hence should optimize the production of ethanol.

Fluorescent nanosensors reveal dynamic pH gradients …

2021-6-17 · Fluorescence within the whole biofilm was observed after 3 min following the addition of the nanosensors, suggesting that the nanosensors enter the biofilm …

Luminescence switching in polymerically confined carbon ...

2021-9-1 · Photo-caged carbon nanoparticles (CNPs) that are non-luminescent under typical microscopic illumination but can be activated by UV light have been synthesized in this work. Negatively charged "bare" CNPs with high luminescence can lose their photoluminescence (PL) when they are chemically crosslinked to a mo

Fluorescent nanosensors reveal dynamic pH gradients …

2021-6-17 · Measuring fluorescence intensity using CLSM revealed a reduction in the fluorescence intensity ratio when the S. mutans biofilm was challenged with 1% glucose (Fig. (Fig.7), 7), while there was only a slight reduction in the fluorescence intensity ratio when distilled water is added. These changes in fluorescence intensity can be visualised in ...

Biofilm formation and granule properties in anaerobic ...

2017-9-15 · Anaerobic biofilm and granule formation at high salinity (10 and 20 g Na + /L) is possible.. UASB reactors process can achieve high removal efficiencies at a salinity as high as 20 g Na + /L.. Potassium (0.7 g/L) alleviates the negative effect of 20 g Na + /L on biofilm formation and UASB performance.. Calcium (1 g/L) has a negative effect on biofilm formation and reactor performance.

Biofilms: Microbial Life on Surfaces

2021-10-22 · Polymicrobic biofilm grown on a stainless steel surface in a laboratory potable water biofilm reactor for 14 days, then stained with 4,6-diamidino-2-phenylindole (DAPI) and examined by epifluorescence microscopy. Bar, 20 μ.

Frontiers | Geobacter Dominates the Inner Layers of a ...

The left side of the red signal corresponds to the outermost environment (ME-FBR medium), whereas the right side of the green signal corresponds to the surface of a fluidized activated carbon particle. This image is representative of one out of 30 …